Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies

Files in This Item:
File Description SizeFormat 
Kumar_et_al_Sci_Rep_2015.pdf4.53 MBAdobe PDFDownload
Title: Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies
Authors: Kumar, Pramod
Satyam, Abhigyan
Fan, Xingliang
Rodriguez, Brian J.
et al.
Permanent link:
Date: 4-Mar-2015
Online since: 2015-04-10T10:22:53Z
Abstract: Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro
Funding Details: Health Research Board
Science Foundation Ireland
Type of material: Journal Article
Publisher: Nature Publishing Group
Journal: Scientific Reports
Volume: 5
Issue: 8729
Start page: 1
End page: 10
Keywords: Tissue engineeringSelf-assemblyCell-sheet tissue engineeringScaffold-free tissue engineeringModular tissue engineeringCollagen
DOI: 10.1038/srep08729
Language: en
Status of Item: Peer reviewed
Appears in Collections:Physics Research Collection

Show full item record

Citations 10

Last Week
Last month
checked on Feb 19, 2019

Page view(s) 50

checked on May 25, 2018

Download(s) 50

checked on May 25, 2018

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.