Sex-specific promoters regulate Dnmt3L expression in mouse germ cells

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Title: Sex-specific promoters regulate Dnmt3L expression in mouse germ cells
Authors: Shovlin, T. C.
Bourc'his, D.
La Salle, S.
O'Doherty, Alan
et al.
Permanent link: http://hdl.handle.net/10197/6540
Date: Feb-2007
Abstract: Background: Dnmt3L, a member of the DNA methyltransferase 3 family, lacks enzymatic activity but is required for de-novo methylation of imprinted genes in oocytes and for transposon repression in male germ cells. Methods: We used northern blots, RT-PCR, 5' rapid amplification of complementary DNA (cDNA) ends (RACE), RNase H mapping, real-time/quantitative RT-PCR and in situ hybridization to identify and characterize Dnmt3L transcripts produced during germ cell development. Results: Mouse Dnmt3L uses three sex-specific promoters, not the single promoter previously thought. A promoter active in prospermatogonia drives transcription of an mRNA encoding the full-length protein in perinatal testis, where de-novo methylation occurs. Late pachytene spermatocytes activate a second promoter in intron 9 of the Dnmt3L gene. After this stage, the predominant transcripts are three truncated mRNAs, which appear to be non-coding. We could also detect similar adult testis transcripts in humans. In the mouse ovary, an oocyte-specific promoter located in an intron of the neighbouring autoimmune regulator (Aire) gene produces a transcript with the full open reading frame (ORF). This is the only Dnmt3L transcript found in growing oocytes and is absent in the oocytes of Dnmt3L-/- females. Conclusions: Sex-specific promoters control Dnmt3L expression in the mouse germ line, mirroring the situation at the Dnmt1 and Dnmt3A loci.
Type of material: Journal Article
Publisher: Oxford University Press
Copyright (published version): 2006 the Authors
Keywords: Dnmt3L;Imprinting;Oocyte;Promoter;Testis
DOI: 10.1093/humrep/del379
Language: en
Status of Item: Peer reviewed
Appears in Collections:Medicine Research Collection

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