High-content analysis for drug delivery and nanoparticle applications
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|Title:||High-content analysis for drug delivery and nanoparticle applications||Authors:||Brayden, David James
Dawson, Kenneth A.
O'Brien, Peter J.
Simpson, Jeremy C.
|Permanent link:||http://hdl.handle.net/10197/6636||Date:||Aug-2015||Online since:||2015-06-27T18:37:41Z||Abstract:||High-content analysis (HCA) provides quantitative multiparametric cellular fluorescence data. From its origins in discovery toxicology, it is now addressing fundamental questions in drug delivery. Nanoparticles (NPs), polymers, and intestinal permeation enhancers are being harnessed in drug delivery systems to modulate plasma membrane properties and the intracellular environment. Identifying comparative mechanistic cytotoxicity on sublethal events is crucial to expedite the development of such systems. NP uptake and intracellular routing pathways are also being dissected using chemical and genetic perturbations, with the potential to assess the intracellular fate of targeted and untargeted particles in vitro. As we discuss here, HCA is set to make a major impact in preclinical delivery research by elucidating the intracellular pathways of NPs and the in vitro mechanistic-based toxicology of formulation constituents.||Funding Details:||Department of Agriculture, Food and the Marine
European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland
|Type of material:||Journal Article||Publisher:||Elsevier||Journal:||Drug Discovery Today||Volume:||20||Issue:||8||Start page:||942||End page:||957||Copyright (published version):||2015 Elsevier||Keywords:||Cellular toxicology; Drug delivery vehicles; Nanoparticle intracellular routing; Small interfering RNA (siRNA)||DOI:||10.1016/j.drudis.2015.04.001||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Centre for Bionano Interactions (CBNI) Research Collection|
Conway Institute Research Collection
Veterinary Medicine Research Collection
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