High-content analysis for drug delivery and nanoparticle applications

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Title: High-content analysis for drug delivery and nanoparticle applications
Authors: Brayden, David James
Cryan, Sally-Ann
Dawson, Kenneth A.
O'Brien, Peter J.
Simpson, Jeremy C.
Permanent link: http://hdl.handle.net/10197/6636
Date: Aug-2015
Online since: 2015-06-27T18:37:41Z
Abstract: High-content analysis (HCA) provides quantitative multiparametric cellular fluorescence data. From its origins in discovery toxicology, it is now addressing fundamental questions in drug delivery. Nanoparticles (NPs), polymers, and intestinal permeation enhancers are being harnessed in drug delivery systems to modulate plasma membrane properties and the intracellular environment. Identifying comparative mechanistic cytotoxicity on sublethal events is crucial to expedite the development of such systems. NP uptake and intracellular routing pathways are also being dissected using chemical and genetic perturbations, with the potential to assess the intracellular fate of targeted and untargeted particles in vitro. As we discuss here, HCA is set to make a major impact in preclinical delivery research by elucidating the intracellular pathways of NPs and the in vitro mechanistic-based toxicology of formulation constituents.
Funding Details: Department of Agriculture, Food and the Marine
European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland
Type of material: Journal Article
Publisher: Elsevier
Journal: Drug Discovery Today
Volume: 20
Issue: 8
Start page: 942
End page: 957
Copyright (published version): 2015 Elsevier
Keywords: Cellular toxicologyDrug delivery vehiclesNanoparticle intracellular routingSmall interfering RNA (siRNA)
DOI: 10.1016/j.drudis.2015.04.001
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Conway Institute Research Collection
Veterinary Medicine Research Collection

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