Antifouling activity of enzyme-functionalized silica nanobeads

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Title: Antifouling activity of enzyme-functionalized silica nanobeads
Authors: Zanoni, Michelle
Habimana, Olivier
Amadio, Jessica
Casey, Eoin
Permanent link: http://hdl.handle.net/10197/7148
Date: Mar-2016
Abstract: The amelioration of biofouling in industrial processing equipment is critical for performance and reliability. While conventional biocides are effective in biofouling control, they are potentially hazardous to the environment and in some cases corrosive to materials. Enzymatic approaches have been shown to be effective and can overcome the disadvantages of traditional biocides, however they are typically uneconomic for routine biofouling control. The aim of this study was to design a robust and reusable enzyme-functionalized nano-bead system having biofilm dispersion properties. This work describes the biochemical covalent functionalization of silica-based nanobeads (hereafter referred to as Si-NanoB) with Proteinase K (PK). Results showed that PK-functionalized Si-NanoB are effective in dispersing both protein-based model biofilms and structurally altering Pseudomonas fluorescens biofilms, with significant decreases in surface coverage and thickness of 30.1% and 38.85%, respectively, while increasing surface roughness by 19 % following 24 h treatments on bacterial biofilms. This study shows that enzyme-functionalized nanobeads may potentially be an environmentally friendly and cost effective alternative to pure enzyme and chemical treatments.
Funding Details: European Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Copyright (published version): 2015 the Authors
Keywords: Nanoparticle;Biofilm;Anti-fouling;Enzyme
DOI: 10.1002/bit.25835
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

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