Sequence embedding for fast construction of guide trees for multiple sequence alignment
|Title:||Sequence embedding for fast construction of guide trees for multiple sequence alignment||Authors:||Blackshields, Gordon
Higgins, D. (Des)
|Permanent link:||http://hdl.handle.net/10197/7307||Date:||14-May-2010||Abstract:||The most widely used multiple sequence alignment methods require sequences to be clustered as an initial step. Most sequence clustering methods require a full distance matrix to be computed between all pairs of sequences. This requires memory and time proportional to N2 for N sequences. When N grows larger than 10,000 or so, this becomes increasingly prohibitive and can form a significant barrier to carrying out very large multiple alignments. In this paper, we have tested variations on a class of embedding methods that have been designed for clustering large numbers of complex objects where the individual distance calculations are expensive. These methods involve embedding the sequences in a space where the similarities within a set of sequences can be closely approximated without having to compute all pair-wise distances. We show how this approach greatly reduces computation time and memory requirements for clustering large numbers of sequences and demonstrate the quality of the clusterings by benchmarking them as guide trees for multiple alignment. Source code is available for download from http://www.clustal.org/mbed.tgz.||Funding Details:||Science Foundation Ireland||Type of material:||Journal Article||Publisher:||BioMed Central||Journal:||Algorithms for Molecular Biology||Volume:||5||Issue:||21||Start page:||1||End page:||11||Copyright (published version):||2010 the Authors||Keywords:||DNA sequencing; Input sequences; Embedding; mBed||DOI:||10.1186/1748-7188-5-21||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Conway Institute Research Collection|
Medicine Research Collection
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