Mechanochemical Stimulation of MCF7 Cells with Rod-Shaped Fe-Au Janus Particles Induces Cell Death through Paradoxical Hyperactivation of ERK

DC FieldValueLanguage
dc.contributor.authorKilinc, Devrim
dc.contributor.authorLesniak, Anna
dc.contributor.authorRashdan, Suad Ahmed
dc.contributor.authorGandhi, Dhruv
dc.contributor.authorBlasiak, Agata
dc.contributor.authorFannin, Paul C.
dc.contributor.authorKriegsheim, Alexander von
dc.contributor.authorKolch, Walter
dc.contributor.authorLee, Gil U.
dc.date.accessioned2016-04-08T09:15:35Z
dc.date.available2016-04-08T09:15:35Z
dc.date.copyright2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen
dc.date.issued2015-02-18
dc.identifier.citationAdvanced Healthcare Materialsen
dc.identifier.urihttp://hdl.handle.net/10197/7553
dc.description.abstractMultifunctional nanoparticles that actively target-specific tissues are studied for cancer diagnosis and treatment. Magnetically and optically active particles are of particular interest because they enable multiple imaging modalities and physically modulated therapies, such as magnetic hyperthermia. Fe–Au nanorods are synthesized that have a long iron segment, coated with polyethylene glycol, and a short gold tip functionalized with heregulin (HRG), a known ligand of ErbB family of receptors. HRG–nanorods preferentially target MCF7 cells relative to MDA-MB-231 cells, as demonstrated in a novel microfluidics device. Targeting rates of these classical breast cancer cells correlate with their differential expression of ErbB2/3 receptors. HRG–nanorod binding stimulates the extracellular signal-regulated kinase 1/2 (ERK) phosphorylation in MCF7 cells. The increase in ERK phosphorylation is linked to 'active zones,' dynamic regions in the cell periphery, which exhibit higher rates of particle binding than the rest of the cell. Periodically stretching cells using magnetic tweezers further activates ERK, which leads to cell death in cells co-treated with B-Raf inhibitors, through ERK hyperactivation. Although to a lesser extent, cell death is also achieved through magnetic hyperthermia. These results demonstrate nanoscale targeting and localized mechanochemical treatment of specific cancer cell lines based on their receptor expression using multifunctional nanoparticles.en
dc.description.sponsorshipScience Foundation Irelanden
dc.language.isoenen
dc.publisherWileyen
dc.rightsThis is the author's version of the following article: Devrim Kilinc, Anna Lesniak, Suad A. Rashdan, Dhruv Gandhi, Agata Blasiak, Paul C. Fannin, Alex von Kriegsheim, Walter Kolch, and Gil U. Lee (2014) "Mechanochemical Stimulation of MCF7 Cells with Rod-Shaped Fe-Au Janus Particles Induces Cell Death through Paradoxical Hyperactivation of ERK" Advanced Healthcare Materials, 4(3) : 395-404 which has been published in final form at http://dx.doi.org/10.1002/adhm.201400391.en
dc.subjectActive targetingen
dc.subjectCanceren
dc.subjectErbB receptorsen
dc.subjectHeregulinen
dc.subjectMagnetic hyperthermiaen
dc.titleMechanochemical Stimulation of MCF7 Cells with Rod-Shaped Fe-Au Janus Particles Induces Cell Death through Paradoxical Hyperactivation of ERKen
dc.typeJournal Articleen
dc.statusPeer revieweden
dc.identifier.volume4en
dc.identifier.issue3en
dc.identifier.startpage395en
dc.identifier.endpage404en
dc.identifier.doi10.1002/adhm.201400391-
dc.neeo.contributorKilinc|Devrim|aut|-
dc.neeo.contributorLesniak|Anna|aut|-
dc.neeo.contributorRashdan|Suad Ahmed|aut|-
dc.neeo.contributorGandhi|Dhruv|aut|-
dc.neeo.contributorBlasiak|Agata|aut|-
dc.neeo.contributorFannin|Paul C.|aut|-
dc.neeo.contributorKriegsheim|Alexander von|aut|-
dc.neeo.contributorKolch|Walter|aut|-
dc.neeo.contributorLee|Gil U.|aut|-
dc.description.othersponsorshipNanoremedies Programmeen
dc.description.othersponsorshipProgramme for Research in Third-Level Institutionsen
dc.description.othersponsorshipEuropean Regional Development Funden
dc.description.othersponsorshipErasmus Mundus Gulf Countries Postdoctoral Fellowshipen
dc.description.othersponsorshipMarie Curie Intra-European Fellowshipen
dc.internal.rmsid700055361
dc.date.updated2016-02-22
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/en
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:Conway Institute Research Collection
SBI Research Collection
Medicine Research Collection
Chemistry Research Collection
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