Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production

Files in This Item:
File Description SizeFormat 
Sweeney_et_al_2016_AMAB_accepted_manuscript.pdf670.03 kBAdobe PDFDownload
Title: Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production
Authors: Sweeney, Paul
Murphy, Cormac D.
Caffrey, Patrick
Permanent link: http://hdl.handle.net/10197/7578
Date: Feb-2016
Abstract: The genome of the amphotericin producer Streptomyces nodosus was sequenced. A single scaffold of 7,714,110 bp was obtained. Biosynthetic genes were identified for several natural products including polyketides, peptides, siderophores and terpenes. The majority of these clusters specified known compounds. Most were silent or expressed at low levels and unlikely to compete with amphotericin production. Biosynthesis of a skyllamycin analogue was activated by introducing expression plasmids containing either a gene for a LuxR transcriptional regulator or genes for synthesis of the acyl moiety of the lipopeptide. In an attempt to boost amphotericin production, genes for acyl CoA carboxylases, a phosphopantetheinyl transferase and the AmphRIV transcriptional activator were overexpressed, and the effects on yields were investigated. This study provides the groundwork for metabolic engineering of S. nodosus strains to produce high yields of amphotericin analogues.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Springer
Copyright (published version): 2015 Springer-Verlag Berlin Heidelberg
Keywords: Streptomyces nodosusGenome sequenceAmphotericin B
DOI: 10.1007/s00253-015-7060-9
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biomolecular and Biomedical Science Research Collection

Show full item record

Citations 50

Last Week
Last month
checked on Aug 17, 2018

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.