Inhibition of the Pim1 Oncogene Results in Diminished Visual Function

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Title: Inhibition of the Pim1 Oncogene Results in Diminished Visual Function
Authors: Yin, Jun
Shine, Lisa
Raycroft, Francis
Deeti, Sudhakar
Reynolds, Alison
Glaviano, Antonino
O'Farrell, Sean
O'Leary, Olivia
Kilty, Claire
Kennedy, Ciarán
McLoughlin, Sarah
Rice, Megan
Russell, Eileen
Higgins, D. (Des)
Kennedy, Breandán
et al.
Permanent link: http://hdl.handle.net/10197/7751
Date: 26-Dec-2012
Abstract: Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3-5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors results in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.
Funding Details: Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: Public Library of Science
Copyright (published version): 2012 the Authors
Keywords: Genome reannotation;Visual function;Zebrafish model
DOI: 10.1371/journal.pone.0052177
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Biomolecular and Biomedical Science Research Collection
Medicine Research Collection

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