Micromagnet arrays for on-chip focusing, switching, and separation of superparamagnetic beads and single cells

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Title: Micromagnet arrays for on-chip focusing, switching, and separation of superparamagnetic beads and single cells
Authors: Rampini, Stefano
Kilinc, Devrim
Li, Peng
Monteil, C.
Gandhi, Dhruv
Lee, Gil U.
Permanent link: http://hdl.handle.net/10197/7883
Date: 10-Jul-2015
Abstract: Nonlinear magnetophoresis (NLM) is a powerful approach for on-chip transport and separation of superparamagnetic (SPM) beads, based on a travelling magnetic field wave generated by the combination of a micromagnet array (MMA) and an applied rotating magnetic field. Here, we present two novel MMA designs that allow SPM beads to be focused, sorted, and separated on-chip. Converging MMAs were used to rapidly collect the SPM beads from a large region of the chip and focus them into synchronized lines. We characterise the collection efficiency of the devices and demonstrate that they can facilitate on-chip analysis of populations of SPM beads using a single-point optical detector. The diverging MMAs were used to control the transport of the beads and to separate them based on their size. The separation efficiency of these devices was determined by the orientation of the magnetisation of the micromagnets relative to the external magnetic field and the size of the beads relative to that of micromagnets. By controlling these parameters and the rotation of the external magnetic field we demonstrated the controlled transport of SPM bead-labelled single MDA-MB-231 cells. The use of these novel MMAs promises to allow magnetically-labelled cells to be efficiently isolated and then manipulated on-chip for analysis with high-resolution chemical and physical techniques.
Funding Details: European Commission - European Regional Development Fund
Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Journal: Lab on a Chip
Volume: 15
Issue: 16
Start page: 3370
End page: 3379
Copyright (published version): 2015 The Royal Society of Chemistry
Keywords: MicromagnetsMagnetic beadsNonlinear magnetohporesisSeparation
DOI: 10.1039/C5LC00581G
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemistry Research Collection

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