Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate
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|Title:||Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate||Authors:||Sweeney, Joseph
Murphy, Cormac D.
|Permanent link:||http://hdl.handle.net/10197/7963||Date:||Dec-2015||Abstract:||Development of a biosensor for the convenient measurement of acetate and propionate concentrations in a two-phase anaerobic digestor (AD) requires a bacterium that will be unresponsive to the other organic acids present in the leachate, of which lactate is the most abundant. Successive gene knockouts of E.coli W3110 d-lactate dehydrogenase (dld), l-lactate dehydrogenase (lldD), glycolate oxidase (glcD) and a suspected l-lactate dehdrogenase (ykgF) were performed. The resulting quadruple mutant (IMD Wldgy) was incapable of growth on d- and l-lactate, whereas the wild type grew readily on these substrates. Furthermore, the O2 consumption rates of acetate-grown IMD Wldgy cell suspensions supplied with either acetate (0.1 mM) or a synthetic leachate including acetate (0.1 mM) and dl-lactate (1 mM) were identical (2.79 and 2.70 mg l−1 min−1, respectively). This was in marked contrast to similar experiments with the wild type which gave initial O2 consumption rates of 2.00, 2.36 and 2.97 mg l−1 min−1 when cell suspensions were supplied with acetate (0.1 mM), acetate (0.1 mM) plus d-lactate (1 mM) or acetate (0.1 mM) plus l-lactate (1 mM), respectively. The knockout strain provides a platform for the design of a biosensor that can accessibly monitor acetate and propionate concentrations in AD leachate via O2-uptake measurements.||Funding Details:||Science Foundation Ireland||Type of material:||Journal Article||Publisher:||Springer||Copyright (published version):||2015 Springer-Verlag Berlin Heidelberg||Keywords:||Anaerobic digestion;Biosensor;Volatile fatty acids;Synthetic leachate||DOI:||10.1007/s00253-015-6887-4||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Biomolecular and Biomedical Science Research Collection|
Biosystems and Food Engineering Research Collection
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