Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate

Files in This Item:
File Description SizeFormat 
Sweeney_et_al_Revision_July_2015.docx229.39 kBMicrosoft WordDownload
Title: Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate
Authors: Sweeney, Joseph
Murphy, Cormac D.
McDonnell, Kevin
Permanent link: http://hdl.handle.net/10197/7963
Date: Dec-2015
Abstract: Development of a biosensor for the convenient measurement of acetate and propionate concentrations in a two-phase anaerobic digestor (AD) requires a bacterium that will be unresponsive to the other organic acids present in the leachate, of which lactate is the most abundant. Successive gene knockouts of E.coli W3110 d-lactate dehydrogenase (dld), l-lactate dehydrogenase (lldD), glycolate oxidase (glcD) and a suspected l-lactate dehdrogenase (ykgF) were performed. The resulting quadruple mutant (IMD Wldgy) was incapable of growth on d- and l-lactate, whereas the wild type grew readily on these substrates. Furthermore, the O2 consumption rates of acetate-grown IMD Wldgy cell suspensions supplied with either acetate (0.1 mM) or a synthetic leachate including acetate (0.1 mM) and dl-lactate (1 mM) were identical (2.79 and 2.70 mg l−1 min−1, respectively). This was in marked contrast to similar experiments with the wild type which gave initial O2 consumption rates of 2.00, 2.36 and 2.97 mg l−1 min−1 when cell suspensions were supplied with acetate (0.1 mM), acetate (0.1 mM) plus d-lactate (1 mM) or acetate (0.1 mM) plus l-lactate (1 mM), respectively. The knockout strain provides a platform for the design of a biosensor that can accessibly monitor acetate and propionate concentrations in AD leachate via O2-uptake measurements.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Springer
Copyright (published version): 2015 Springer-Verlag Berlin Heidelberg
Keywords: Anaerobic digestion;Biosensor;Volatile fatty acids;Synthetic leachate
DOI: 10.1007/s00253-015-6887-4
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biomolecular and Biomedical Science Research Collection
Biosystems and Food Engineering Research Collection

Show full item record

SCOPUSTM   
Citations 50

1
Last Week
0
Last month
checked on Jun 22, 2018

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.