Comparison of three methods for the detection of Angiostrongylus vasorum in the final host

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Title: Comparison of three methods for the detection of Angiostrongylus vasorum in the final host
Authors: Houpin, E.McCarthy, GrainneFerrand, M.De Waal, TheoO'Neill, Emma J.Zintl, Annetta
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Date: 15-Apr-2016
Online since: 2017-04-15T01:00:13Z
Abstract: Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
Type of material: Journal Article
Publisher: Elsevier
Journal: Veterinary Parasitology
Volume: 220
Start page: 54
End page: 58
Copyright (published version): 2016 Elsevier
Keywords: Angiostrongylus vasorumCrenosoma vulpisVulpes vulpesDiagnosisPost-mortem examinationIDEXX Angio Detect kitNested PCR-RFLP
DOI: 10.1016/j.vetpar.2016.02.023
Language: en
Status of Item: Peer reviewed
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Appears in Collections:Veterinary Medicine Research Collection

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