Comparison of three methods for the detection of Angiostrongylus vasorum in the final host

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Title: Comparison of three methods for the detection of Angiostrongylus vasorum in the final host
Authors: Houpin, E.
McCarthy, Grainne
Ferrand, M.
De Waal, Theo
O'Neill, Emma J.
Zintl, Annetta
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Date: 15-Apr-2016
Abstract: Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
Type of material: Journal Article
Publisher: Elsevier
Copyright (published version): 2016 Elsevier
Keywords: Angiostrongylus vasorum;Crenosoma vulpis;Vulpes vulpes;Diagnosis;Post-mortem examination;IDEXX Angio Detect kit;Nested PCR-RFLP
DOI: 10.1016/j.vetpar.2016.02.023
Language: en
Status of Item: Peer reviewed
Appears in Collections:Veterinary Medicine Research Collection

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