In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry

Title: In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry
Authors: Lo Giudice, Maria Cristina
Herda, Luciana M.
Polo, Ester
Dawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/8197
Date: 15-Nov-2016
Abstract: Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or ‘biomolecular corona’ conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle–biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments.
Funding Details: Science Foundation Ireland
University College Dublin
Type of material: Journal Article
Publisher: Nature Publishing Group
Copyright (published version): 2016 the Authors
Keywords: Nanoparticles;Nanostructures
DOI: 10.1038/ncomms13475
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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