In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry
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|Title:||In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry||Authors:||Lo Giudice, Maria Cristina
Herda, Luciana M.
Dawson, Kenneth A.
|Permanent link:||http://hdl.handle.net/10197/8197||Date:||15-Nov-2016||Abstract:||Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or ‘biomolecular corona’ conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle–biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments.||Funding Details:||Science Foundation Ireland
University College Dublin
|Type of material:||Journal Article||Publisher:||Nature Publishing Group||Copyright (published version):||2016 the Authors||Keywords:||Nanoparticles;Nanostructures||DOI:||10.1038/ncomms13475||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Centre for Bionano Interactions (CBNI) Research Collection|
Chemistry Research Collection
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