Revealing region-specific biofilm viscoelastic properties by means of a microrheological approach
|Title:||Revealing region-specific biofilm viscoelastic properties by means of a microrheological approach||Authors:||Cao, Huayu
|Permanent link:||http://hdl.handle.net/10197/8284||Date:||5-Dec-2016||Abstract:||Particle-tracking microrheology is an in situ technique that allows quantification of biofilm material properties. It overcomes the limitations of alternative techniques such as bulk rheology or force spectroscopy by providing data on region specific material properties at any required biofilm location and can be combined with confocal microscopy and associated structural analysis. This article describes single particle tracking microrheology combined with confocal laser scanning microscopy to resolve the biofilm structure in 3 dimensions and calculate the creep compliances locally. Samples were analysed from Pseudomonas fluorescens biofilms that were cultivated over two timescales (24hr and 48hr) and alternate ionic conditions (with and without calcium chloride supplementation). The region-based creep compliance analysis showed that the creep compliance of biofilm void zones is the primary contributor to biofilm mechanical properties, contributing to the overall viscoelastic character.||Funding Details:||European Research Council
Science Foundation Ireland
|Type of material:||Journal Article||Publisher:||Nature Publishing Group||Copyright (published version):||2016 the Authors||Keywords:||Biofilms; Biological techniques||DOI:||10.1038/s41522-016-0005-y||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Chemical and Bioprocess Engineering Research Collection|
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