Streptomyces nodosus Host Strains Optimised for Glycosylation Engineering

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Title: Streptomyces nodosus Host Strains Optimised for Glycosylation Engineering
Authors: Stephens, Niamh
Rawlings, Bernard
Caffrey, Patrick
Permanent link: http://hdl.handle.net/10197/8302
Date: 2012
Abstract: The AmphDI glycosyltransferase transfers a mycosaminyl sugar residue from GDP onto 8-deoxyamphoteronolide B, the aglycone of the antifungal amphotericin B. In this study the amphDI gene was inactivated in Streptomyces nodosus strains lacking the AmphN cytochrome P450. The new mutants produced 8-deoxy-16-methyl-16-descarboxyl amphoteronolides in high yield. These strains and aglycones should prove valuable for in vivo and in vitro glycosylation engineering.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Taylor and Francis
Keywords: Polyene macrolide biosynthesisGlycosylation engineeringAmphotericin
DOI: 10.1271/bbb.110673
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biomolecular and Biomedical Science Research Collection
CSCB Research Collection

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