Quantifying nanoscale biochemical heterogeneity in human epithelial cancer cells using combined AFM and PTIR absorption nanoimaging

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Title: Quantifying nanoscale biochemical heterogeneity in human epithelial cancer cells using combined AFM and PTIR absorption nanoimaging
Authors: Kennedy, Eamonn
Al-Majmaie, Rasoul
Al-Rubeai, Mohamed
Zerulla, Dominic
Rice, James H.
Permanent link: http://hdl.handle.net/10197/8356
Date: Jan-2015
Abstract: Subcellular chemical heterogeneity plays a key role in cell organization and function. However the biomechanics underlying the structure-function relationship is governed by cell substructures which are poorly resolved using conventional chemical imaging methods. To date, advances in sub-diffraction limited infrared (IR) nanoscopy have permitted intracellular chemical mapping. In this work we report how image analysis applied to a combination of IR absorption nanoimaging and topographic data permits quantification of chemical complexity at the nanoscale, enabling the analysis of biochemical heterogeneity in mammalian cancer cells on the scale of subcellular features.
Type of material: Journal Article
Publisher: Wiley
Copyright (published version): 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords: Atomic force microscopy (AFM);Photothermal induced resonance (PTIR);Infrared spectral imaging;Colon cancer;Nanotechnology;Subcellular imaging
DOI: 10.1002/jbio.201300138
Language: en
Status of Item: Peer reviewed
Appears in Collections:Physics Research Collection
Chemical and Bioprocess Engineering Research Collection

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