Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

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Title: Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility
Authors: Li, Peng
Kilinc, Devrim
Ying, Fen Ran
Lee, Gil U.
Permanent link: http://hdl.handle.net/10197/8378
Date: 20-Aug-2013
Abstract: Magnetic separation provides a rapid and efficient means of isolating biomaterials from complex mixtures based on their adsorption on superparamagnetic (SPM) beads. Flow enhanced non-linear magnetophoresis (FNLM) is a high-resolution mode of separation in which hydrodynamic and magnetic fields are controlled with micron resolution to isolate SPM beads with specific physical properties. In this article we demonstrate that a change in the critical frequency of FNLM can be used to identify beads with magnetic susceptibilities between 0.01 and 1.0 with a sensitivity of 0.01 Hz(-1). We derived an analytical expression for the critical frequency that explicitly incorporates the magnetic and non-magnetic composition of a complex to be separated. This expression was then applied to two cases involving the detection and separation of biological targets. This study defines the operating principles of FNLM and highlights the potential for using this technique for multiplexing diagnostic assays and isolating rare cell types.
Funding Details: Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: Royal Society of Chemistry
Copyright (published version): 2013 The Royal Society of Chemistry
Keywords: Superparamagnetic beadsFlow enhanced non-linear magnetophoresis
DOI: 10.1039/C3LC50816A
Language: en
Status of Item: Peer reviewed
Appears in Collections:Medicine Research Collection

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