Isolation of Bowman-Birk-Inhibitor from soybean extracts using novel peptide probes and high gradient magnetic separation
Files in This Item:
|Food_Chemistry_2012_-_Isolation_of_Bowman.pdf||4.82 MB||Adobe PDF||Download|
|Title:||Isolation of Bowman-Birk-Inhibitor from soybean extracts using novel peptide probes and high gradient magnetic separation||Authors:||Fields, Conor
Lee, Gil U.
|Permanent link:||http://hdl.handle.net/10197/8385||Date:||15-Oct-2012||Abstract:||Soybean proteins offer exceptional promise in the area of cancer prevention and treatment. Specifically, Bowman-Birk Inhibitor (BBI) has the ability to suppress carcinogenesis in vivo, which has been attributed to BBI’s inhibition of serine protease (trypsin and chymotrypsin) activity. The lack of molecular probes for the isolation of this protein has made it difficult to work with, limiting its progress as a significant candidate in the treatment of cancer. This study has successfully identified a set of novel synthetic peptides targeting the BBI, and has demonstrated the ability to bind BBI in vitro. One of those probes has been covalently immobilised on superparamagnetic microbeads to allow the isolation of BBI from soy whey mixtures in a single step. Our ultimate goal is the use of the described synthetic probe to facilitate the isolation of this potentially therapeutic protein for low cost, scalable analysis and production of BBI.||Funding Details:||European Commission - Seventh Framework Programme (FP7)||Type of material:||Journal Article||Publisher:||Elsevier||Journal:||Food Chemistry||Volume:||134||Issue:||4||Start page:||1831||End page:||1838||Copyright (published version):||2012 Elsevier||Keywords:||Soybean proteins; Bowman-Birk Inhibitor; Phage display; Superparamagnetic particles; Purification; Soluble peptide binders||DOI:||10.1016/j.foodchem.2012.03.085||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Medicine Research Collection|
Show full item record
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.