Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation

Files in This Item:
File Description SizeFormat 
Small_2012_-_Affinity_Separation_.pdf6.63 MBAdobe PDFDownload
Title: Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation
Authors: Muzard, Julien
Platt, Mark
Lee, Gil U.
Permanent link:
Date: 6-Aug-2012
Online since: 2017-03-02T18:05:48Z
Abstract: The growth of the biopharmaceutical industry has created a demand for new technologies for the purification of genetically engineered proteins.The efficiency of large-scale, high-gradient magnetic fishing could be improved if magnetic particles offering higher binding capacity and magnetization were available. This article describes several strategies for synthesizing microbeads that are composed of a M13 bacteriophage layer assembled on a superparamagnetic core. Chemically cross-linking the pVIII proteins to a carboxyl functionalized bead produced highly responsive superparamagnetic particles (SPM) with a side-on oriented, adherent virus monolayer. Also, the genetic manipulation of the pIII proteins with a His6 peptide sequence allowed reversible assembly of the bacteriophage on a nitrilotriacetic acid functionalized core in an end-on configuration. These phage-magnetic particles were successfully used to separate antibodies from high-protein concentration solutions in a single step with a > 90 % purity. The dense magnetic core of these particles makes themfive times more responsive to magnetic fields than commercial materialscomposed of polymer-iron oxide compositesand a monolayer of phage could produced a 1000 fold higher antibody binding capacity. These new bionanomaterials appear to be well-suited to large-scale high-gradient magnetic fishing separation and promise to be cost effective as a result of the self-assembling and self-replicating properties of genetically engineered M13 bacteriophage
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Journal: Small
Volume: 8
Issue: 15
Copyright (published version): 2012 Wiley
Keywords: BionanomaterialM13 filamentous phageAffinity separationDownstream processingSuperparamagnetic beadsHigh gradient magnetic separation
DOI: 10.1002/smll.201290083
Language: en
Status of Item: Peer reviewed
Appears in Collections:Medicine Research Collection

Show full item record

Download(s) 50

checked on May 25, 2018

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.