Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation
|Title:||Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation||Authors:||Muzard, Julien; Platt, Mark; Lee, Gil U.||Permanent link:||http://hdl.handle.net/10197/8386||Date:||6-Aug-2012||Online since:||2017-03-02T18:05:48Z||Abstract:||The growth of the biopharmaceutical industry has created a demand for new technologies for the purification of genetically engineered proteins.The efficiency of large-scale, high-gradient magnetic fishing could be improved if magnetic particles offering higher binding capacity and magnetization were available. This article describes several strategies for synthesizing microbeads that are composed of a M13 bacteriophage layer assembled on a superparamagnetic core. Chemically cross-linking the pVIII proteins to a carboxyl functionalized bead produced highly responsive superparamagnetic particles (SPM) with a side-on oriented, adherent virus monolayer. Also, the genetic manipulation of the pIII proteins with a His6 peptide sequence allowed reversible assembly of the bacteriophage on a nitrilotriacetic acid functionalized core in an end-on configuration. These phage-magnetic particles were successfully used to separate antibodies from high-protein concentration solutions in a single step with a > 90 % purity. The dense magnetic core of these particles makes themfive times more responsive to magnetic fields than commercial materialscomposed of polymer-iron oxide compositesand a monolayer of phage could produced a 1000 fold higher antibody binding capacity. These new bionanomaterials appear to be well-suited to large-scale high-gradient magnetic fishing separation and promise to be cost effective as a result of the self-assembling and self-replicating properties of genetically engineered M13 bacteriophage||Funding Details:||Science Foundation Ireland||Funding Details:||Irish National Institute for Bioprocessing Research and Training (NIBRT)
IEF Marie Curie fellowship
|Type of material:||Journal Article||Publisher:||Wiley||Journal:||Small||Volume:||8||Issue:||15||Copyright (published version):||2012 Wiley||Keywords:||Bionanomaterial; M13 filamentous phage; Affinity separation; Downstream processing; Superparamagnetic beads; High gradient magnetic separation||DOI:||10.1002/smll.201290083||Language:||en||Status of Item:||Peer reviewed||This item is made available under a Creative Commons License:||https://creativecommons.org/licenses/by-nc-nd/3.0/ie/|
|Appears in Collections:||Medicine Research Collection|
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