A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding
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|Title:||A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding||Authors:||Vartia, Salla
Villanueva-Cañas, José L.
Finarelli, John A.
Farrell, Edward D.
Hughes, Graham M.
Carlsson, Jeanette E. L.
|Permanent link:||http://hdl.handle.net/10197/8467||Date:||20-Jan-2016||Abstract:||This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing'(GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS of microsatellites, using individual combinatorial barcoding, is potentially faster and cheaper than current microsatellite approaches and offers better and more data.||Funding Details:||European Commission - European Regional Development Fund
Irish Research Council
Science Foundation Ireland
|Type of material:||Journal Article||Publisher:||The Royal Society||Copyright (published version):||2016 the Authors||Keywords:||Amplicon sequencin;Gadus morhua;Genotyping by sequencing;Next-generation;Sequencing;Ssr;Universal primer||DOI:||10.1098/rsos.150565||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Biology & Environmental Science Research Collection|
Earth Institute Research Collection
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