A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding

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Title: A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding
Authors: Vartia, Salla
Villanueva-Cañas, José L.
Finarelli, John A.
Farrell, Edward D.
Hughes, Graham M.
Carlsson, Jeanette E. L.
Carlsson, Jens
et al.
Permanent link: http://hdl.handle.net/10197/8467
Date: 20-Jan-2016
Abstract: This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing'(GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS of microsatellites, using individual combinatorial barcoding, is potentially faster and cheaper than current microsatellite approaches and offers better and more data.
Funding Details: European Commission - European Regional Development Fund
Irish Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: The Royal Society
Copyright (published version): 2016 the Authors
Keywords: Amplicon sequencin;Gadus morhua;Genotyping by sequencing;Next-generation;Sequencing;Ssr;Universal primer
DOI: 10.1098/rsos.150565
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biology & Environmental Science Research Collection
Earth Institute Research Collection

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