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  5. Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles
 
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Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles

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Download Luciana_-_JACS_paper.pdf1.2 MB
Author(s)
Herda, Luciana M. 
Hristov, Delyan R. 
Lo Giudice, Maria Cristina 
Polo, Ester 
Dawson, Kenneth A. 
Uri
http://hdl.handle.net/10197/9171
Date Issued
22 December 2016
Date Available
16T11:29:04Z January 2018
Abstract
Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and in vivo targeting, though an important feature of such a system, has proved challenging. Although extensive physicochemical characterization of targeting nanoparticles can be addressed in detail, relevant biological characterization of the nanointerface is crucial in order to select suitable nanomaterials for further in vitro or in vivo experiments. In this work, we adopt a methodology using antibody fragments (Fab) conjugated to gold nanoparticles (immunogold) to map the available epitopes on a transferrin grafted silica particle (SiO2−PEG8−Tf) as a proxy methodology to predict nanoparticle biological function, and therefore cellular receptor engagement. Data from the adopted method suggest that, on average, only∼3.5% of proteins grafted on the SiO2−PEG8−Tf nanoparticle surface have a favorable orientation for recognition by the cellular receptor.
Sponsorship
European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland
Type of Material
Journal Article
Publisher
ACS
Journal
Journal of the American Chemical Society
Volume
139
Issue
1
Start Page
111
End Page
114
Copyright (Published Version)
2016 ACS
Keywords
  • Nanomedicine

  • Nanoparticle design

DOI
10.1021/jacs.6b12297
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Owning collection
Chemistry Research Collection
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