Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles

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Title: Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles
Authors: Herda, Luciana M.
Hristov, Delyan R.
Lo Giudice, Maria Cristina
Polo, Ester
Dawson, Kenneth A.
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Date: 22-Dec-2016
Abstract: Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and in vivo targeting, though an important feature of such a system, has proved challenging. Although extensive physicochemical characterization of targeting nanoparticles can be addressed in detail, relevant biological characterization of the nanointerface is crucial in order to select suitable nanomaterials for further in vitro or in vivo experiments. In this work, we adopt a methodology using antibody fragments (Fab) conjugated to gold nanoparticles (immunogold) to map the available epitopes on a transferrin grafted silica particle (SiO2−PEG8−Tf) as a proxy methodology to predict nanoparticle biological function, and therefore cellular receptor engagement. Data from the adopted method suggest that, on average, only∼3.5% of proteins grafted on the SiO2−PEG8−Tf nanoparticle surface have a favorable orientation for recognition by the cellular receptor.
Funding Details: European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland
Type of material: Journal Article
Publisher: ACS
Copyright (published version): 2016 ACS
Keywords: NanomedicineNanoparticle design
DOI: 10.1021/jacs.6b12297
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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