Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles

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Title: Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles
Authors: Lara, Sandra
Alnasser, Fatima
Polo, Ester
Garry, David
Lo Giudice, Maria Cristina
Hristov, Delyan R.
Rocks, Louise
Salvati, Anna
Yan, Yan
Dawson, Kenneth A.
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Date: 23-Jan-2017
Online since: 2018-01-23T02:00:13Z
Abstract: Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. Our results suggest that the “labeling” of nanoparticles by biomolecular adsorption processes allows for multiple pathways in biological processes in which they may be “mistaken” for endogenous objects, such as lipoproteins, and exogenous ones, such as viral infections.
Funding Details: European Commission - Seventh Framework Programme (FP7)
Science Foundation Ireland
Type of material: Journal Article
Publisher: ACS
Journal: ACS Nano
Volume: 11
Issue: 2
Start page: 1884
End page: 1893
Copyright (published version): 2017 ACS
Keywords: Biomolecular coronaEpitopeInternalizationLow-density lipoproteinNanoparticleReceptor
DOI: 10.1021/acsnano.6b07933
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemistry Research Collection

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