Proteome-wide association studies identify biochemical modules associated with a wing size phenotype in Drosophila melanogaster

Title: Proteome-wide association studies identify biochemical modules associated with a wing size phenotype in Drosophila melanogaster
Authors: Okada, Hirokazu
Ebhardt, H. Alexander
Vonesch, Sibylle Chantal
Aebersold, Ruedi
Hafen, Ernst
Permanent link: http://hdl.handle.net/10197/9201
Date: 1-Sep-2016
Abstract: The manner by which genetic diversity within a population generates individual phenotypes is a fundamental question of biology. To advance the understanding of the genotype–phenotype relationships towards the level of biochemical processes, we perform a proteome-wide association study (PWAS) of a complex quantitative phenotype. We quantify the variation of wing imaginal disc proteomes in Drosophila genetic reference panel (DGRP) lines using SWATH mass spectrometry. In spite of the very large genetic variation (1/36 bp) between the lines, proteome variability is surprisingly small, indicating strong molecular resilience of protein expression patterns. Proteins associated with adult wing size form tight co-variation clusters that are enriched in fundamental biochemical processes. Wing size correlates with some basic metabolic functions, positively with glucose metabolism but negatively with mitochondrial respiration and not with ribosome biogenesis. Our study highlights the power of PWAS to filter functional variants from the large genetic variability in natural populations.
Funding Details: European Research Council
Type of material: Journal Article
Publisher: Springer Nature
Copyright (published version): 2016 the Authors
Keywords: PWAS;Proteome;Genetic diversity
DOI: 10.1038/ncomms12649
Language: en
Status of Item: Peer reviewed
Appears in Collections:SBI Research Collection
Medicine Research Collection

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