Neuronal Cell Bodies Remotely Regulate Axonal Growth Response to Localized Netrin-1 Treatment via Second Messenger and DCC Dynamics

DC FieldValueLanguage
dc.contributor.authorBlasiak, Agata-
dc.contributor.authorKilinc, Devrim-
dc.contributor.authorLee, Gil U.-
dc.date.accessioned2019-01-30T16:10:16Z-
dc.date.available2019-01-30T16:10:16Z-
dc.date.copyright2018 the Authorsen_US
dc.date.issued2017-01-05-
dc.identifier.citationFrontiers in Cellular Neuroscienceen_US
dc.identifier.issn1662-5102-
dc.identifier.urihttp://hdl.handle.net/10197/9605-
dc.description.abstractNetrin-1 modulates axonal growth direction and speed. Its best characterized receptor, Deleted in Colorectal Cancer (DCC), is localized to growth cones, but also observed in the cell bodies. We hypothesized that cell bodies sense Netrin-1 and contribute to axon growth rate modulation, mediated by the second messenger system. We cultured mouse cortical neurons in microfluidic devices to isolate distal axon and cell body microenvironments. Compared to isolated axonal treatment, global Netrin-1 treatment decreased the axon elongation rate and affected the dynamics of total and membranous DCC, calcium, and cyclic nucleotides. Signals induced by locally applied Netrin-1 propagated in both anterograde and retrograde directions, demonstrated by the long-range increase in DCC and by the increased frequency of calcium transients in cell bodies, evoked by axonal Netrin-1. Blocking the calcium efflux from endoplasmic reticulum suppressed the membranous DCC response. Our findings support the notion that neurons sense Netrin-1 along their entire lengths in making axonal growth decisions.en_US
dc.description.sponsorshipEuropean Commission - European Regional Development Funden_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.format.mediumElectronic-eCollection-
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectMicrofluidicsen_US
dc.subjectPath findingen_US
dc.subjectGuidance cuesen_US
dc.subjectCompartmentalizationen_US
dc.subjectCalcium signalingen_US
dc.titleNeuronal Cell Bodies Remotely Regulate Axonal Growth Response to Localized Netrin-1 Treatment via Second Messenger and DCC Dynamicsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactothergil.lee@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume10en_US
dc.identifier.issue298en_US
dc.identifier.doi10.3389/fncel.2016.00298-
dc.neeo.contributorBlasiak|Agata|aut|-
dc.neeo.contributorKilinc|Devrim|aut|-
dc.neeo.contributorLee|Gil U.|aut|-
dc.description.othersponsorshipNanoremedies Programmeen_US
dc.description.othersponsorshipMarie-Curie Intra-European Fellowshipen_US
dc.description.othersponsorshipAXA Research Fund Doctoral Fellowshipen_US
dc.date.updated2018-11-13T11:18:25Z-
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Conway Institute Research Collection
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