Plasma EBV microRNAs in paediatric renal transplant recipients

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Title: Plasma EBV microRNAs in paediatric renal transplant recipients
Authors: Hassan, Jaythoon
Dean, Jonathan
De Gascun, Cillian
Connell, Jeff
et al.
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Date: 28-Nov-2017
Online since: 2019-03-22T12:36:56Z
Abstract: Background: Epstein-Barr virus (EBV) was the first human virus identified to express microRNA (miRNA). To date, 44mature miRNAs are encoded for within the EBV genome. EBV miRNAs have not been profiled in paediatric renal transplant recipients. In this study, we investigated circulating EBV miRNA profiles as novel biomarkers in paediatric renal transplant patients. Methods: Forty-two microRNAs encoded within 2 EBV open reading frames (BART and BHRF) were examined in renal transplant recipients who resolved EBV infection (REI) or maintained chronic high viral loads (CHL), and in non-transplant patients with acute infectious mononucleosis (IM). Results: Plasma EBV-miR-BART2-5p was present in higher numbers of IM (7/8) and CHL (7/10) compared to REI (7/12)patients. A trend was observed between the numbers of plasma EBV miRNAs expressed and EBV viral load (p < 0.07).Several EBV-miRs including BART7-3p, 15, 9-3p, 11-3p, 1-3p and 3-3p were detected in IM and CHL patients only. Thelytic EBV-miRs, BHRF1-2-3p and 1-1, indicating active viral replication, were detected in IM patients only. One CHL patient developed post-transplant lymphoproliferative disease (PTLD) after several years and analysis of 10 samples over a 30-month period showed an average 24-fold higher change in plasma EBV-miR-BART2-5p compared to the CHL group and 110-fold higher change compared to the REI group.Conclusions Our results suggest that EBV-miR-BART2-5p, which targets the stress-induced immune ligand MICB to escape recognition and elimination by NK cells, may have a role in sustaining high EBV viral loads in CHL paediatric kidney transplant recipients.
Type of material: Journal Article
Publisher: Springer
Journal: Journal of Nephrology
Volume: 31
Issue: 3
Start page: 445
End page: 451
Copyright (published version): 2017 Italian Society of Nephrology
Keywords: EBV miRNARenal transplant recipientsPlasma EBV-miR-BARt2-5pEBV viral load
DOI: 10.1007/s40620-017-0462-2
Language: en
Status of Item: Peer reviewed
Appears in Collections:UCD National Virus Reference Laboratory Research Collection

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