Plasma EBV microRNAs in paediatric renal transplant recipients

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dc.contributor.authorHassan, Jaythoon-
dc.contributor.authorDean, Jonathan-
dc.contributor.authorDe Gascun, Cillian-
dc.contributor.authorConnell, Jeff-
dc.contributor.authoret al.- Italian Society of Nephrologyen_US
dc.identifier.citationJournal of Nephrologyen_US
dc.description.abstractBackground: Epstein-Barr virus (EBV) was the first human virus identified to express microRNA (miRNA). To date, 44mature miRNAs are encoded for within the EBV genome. EBV miRNAs have not been profiled in paediatric renal transplant recipients. In this study, we investigated circulating EBV miRNA profiles as novel biomarkers in paediatric renal transplant patients. Methods: Forty-two microRNAs encoded within 2 EBV open reading frames (BART and BHRF) were examined in renal transplant recipients who resolved EBV infection (REI) or maintained chronic high viral loads (CHL), and in non-transplant patients with acute infectious mononucleosis (IM). Results: Plasma EBV-miR-BART2-5p was present in higher numbers of IM (7/8) and CHL (7/10) compared to REI (7/12)patients. A trend was observed between the numbers of plasma EBV miRNAs expressed and EBV viral load (p < 0.07).Several EBV-miRs including BART7-3p, 15, 9-3p, 11-3p, 1-3p and 3-3p were detected in IM and CHL patients only. Thelytic EBV-miRs, BHRF1-2-3p and 1-1, indicating active viral replication, were detected in IM patients only. One CHL patient developed post-transplant lymphoproliferative disease (PTLD) after several years and analysis of 10 samples over a 30-month period showed an average 24-fold higher change in plasma EBV-miR-BART2-5p compared to the CHL group and 110-fold higher change compared to the REI group.Conclusions Our results suggest that EBV-miR-BART2-5p, which targets the stress-induced immune ligand MICB to escape recognition and elimination by NK cells, may have a role in sustaining high EBV viral loads in CHL paediatric kidney transplant recipients.en_US
dc.subjectEBV miRNAen_US
dc.subjectRenal transplant recipientsen_US
dc.subjectPlasma EBV-miR-BARt2-5pen_US
dc.subjectEBV viral loaden_US
dc.titlePlasma EBV microRNAs in paediatric renal transplant recipientsen_US
dc.typeJournal Articleen_US
dc.statusPeer revieweden_US
dc.neeo.contributorDe Gascun|Cillian|aut|-
dc.neeo.contributoret al.||aut|-
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