Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Sploceosome Activation in Fission Yeast
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|Title:||Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Sploceosome Activation in Fission Yeast||Authors:||Patrick, Kristin L.
Ryan, Colm J.
|Permanent link:||http://hdl.handle.net/10197/9779||Date:||31-Mar-2015||Online since:||2019-04-03T08:04:11Z||Abstract:||Although numerous regulatory connections between pre-mRNA splicing and chromatin have been demonstrated, the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. To probe the genetic network of pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe, we constructed an epistatic mini-array profile (E-MAP) and discovered many new connections between chromatin and splicing. Notably, the nucleosome remodeler SWI/SNF had strong genetic interactions with components of the U2 snRNP SF3 complex. Overexpression of SF3 components in ΔSWI/SNF cells led to inefficient splicing of many fission yeast introns, predominantly those with non-consensus splice sites. Deletion of SWI/SNF decreased recruitment of the splicing ATPase Prp2, suggesting that SWI/SNF promotes co-transcriptional spliceosome assembly prior to first step catalysis. Importantly, defects in SWI/SNF as well as SF3 overexpression each altered nucleosome occupancy along intron-containing genes, illustrating that the chromatin landscape both affects--and is affected by--co-transcriptional splicing.||Type of material:||Journal Article||Publisher:||Public Library of Science||Journal:||PLOS Genetics||Volume:||11||Issue:||3||Start page:||1||End page:||25||Copyright (published version):||2015 the Authors||Keywords:||Genetic interaction mapping; SWI/SNF; Spliceosome activation; Fission Yeast; Pre-mRNA splicing; E-MAP; SF3; ATPase Prp2; Genome-wide genetic interaction map||DOI:||10.1371/journal.pgen.1005074||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Computer Science Research Collection|
SBI Research Collection
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