Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Sploceosome Activation in Fission Yeast

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Title: Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Sploceosome Activation in Fission Yeast
Authors: Patrick, Kristin L.
Ryan, Colm J.
Xu, Jiewei
et al.
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Date: 31-Mar-2015
Online since: 2019-04-03T08:04:11Z
Abstract: Although numerous regulatory connections between pre-mRNA splicing and chromatin have been demonstrated, the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. To probe the genetic network of pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe, we constructed an epistatic mini-array profile (E-MAP) and discovered many new connections between chromatin and splicing. Notably, the nucleosome remodeler SWI/SNF had strong genetic interactions with components of the U2 snRNP SF3 complex. Overexpression of SF3 components in ΔSWI/SNF cells led to inefficient splicing of many fission yeast introns, predominantly those with non-consensus splice sites. Deletion of SWI/SNF decreased recruitment of the splicing ATPase Prp2, suggesting that SWI/SNF promotes co-transcriptional spliceosome assembly prior to first step catalysis. Importantly, defects in SWI/SNF as well as SF3 overexpression each altered nucleosome occupancy along intron-containing genes, illustrating that the chromatin landscape both affects--and is affected by--co-transcriptional splicing.
Type of material: Journal Article
Publisher: Public Library of Science
Journal: PLOS Genetics
Volume: 11
Issue: 3
Start page: 1
End page: 25
Copyright (published version): 2015 the Authors
Keywords: Genetic interaction mappingSWI/SNFSpliceosome activationFission YeastPre-mRNA splicingE-MAPSF3ATPase Prp2Genome-wide genetic interaction map
DOI: 10.1371/journal.pgen.1005074
Language: en
Status of Item: Peer reviewed
Appears in Collections:Computer Science Research Collection
SBI Research Collection

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