Welcome to Research Repository UCD
Research Repository UCD is a digital collection of open access scholarly research publications from University College Dublin. Research Repository UCD collects, preserves and makes freely available publications including peer-reviewed articles, working papers and conference papers created by UCD researchers. Where material has already been published it is made available subject to the open-access policies of the original publishers. This service is maintained by UCD Library.
Comparison of in vitro viability methods for Cryptosporidium oocysts
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Vande_Burgt_et_al_2018.doc | 438.5 kB | Microsoft Word | Download |
| Title: | Comparison of in vitro viability methods for Cryptosporidium oocysts | Authors: | Vande Burgt, Nathan H. Auer, Agathe Zintl, Annetta |
Permanent link: | http://hdl.handle.net/10197/9922 | Date: | Apr-2018 | Online since: | 2019-04-11T11:58:31Z | Abstract: | The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts. | Type of material: | Journal Article | Publisher: | Elsevier | Journal: | Experimental Parasitology | Volume: | 187 | Start page: | 30 | End page: | 36 | Copyright (published version): | 2018 Elsevier | Keywords: | PMA-qPCR; Polymer adhesion; Fluorescence in-situ hybridization; Excystation; Propidium iodide | DOI: | 10.1016/j.exppara.2018.03.002 | Language: | en | Status of Item: | Peer reviewed |
| Appears in Collections: | Veterinary Medicine Research Collection |
Show full item record
SCOPUSTM
Citations
50
2
Last Week
0
0
Last month
checked on May 19, 2019
Google ScholarTM
Check
Altmetric
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.