Comparison of in vitro viability methods for Cryptosporidium oocysts

Files in This Item:
 File SizeFormat
DownloadVande_Burgt_et_al_2018.doc438.5 kBMicrosoft Word
Title: Comparison of in vitro viability methods for Cryptosporidium oocysts
Authors: Vande Burgt, Nathan H.Auer, AgatheZintl, Annetta
Permanent link: http://hdl.handle.net/10197/9922
Date: Apr-2018
Online since: 2019-04-11T11:58:31Z
Abstract: The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts.
Funding Details: Department of Agriculture, Food and the Marine
Type of material: Journal Article
Publisher: Elsevier
Journal: Experimental Parasitology
Volume: 187
Start page: 30
End page: 36
Copyright (published version): 2018 Elsevier
Keywords: PMA-qPCRPolymer adhesionFluorescence in-situ hybridizationExcystationPropidium iodide
DOI: 10.1016/j.exppara.2018.03.002
Language: en
Status of Item: Peer reviewed
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Veterinary Medicine Research Collection

Show full item record

SCOPUSTM   
Citations 50

4
Last Week
0
Last month
0
checked on Sep 12, 2020

Page view(s)

694
Last Week
2
Last month
15
checked on Jun 25, 2022

Download(s)

268
checked on Jun 25, 2022

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.