Comparison of in vitro viability methods for Cryptosporidium oocysts

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Title: Comparison of in vitro viability methods for Cryptosporidium oocysts
Authors: Vande Burgt, Nathan H.Auer, AgatheZintl, Annetta
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Date: Apr-2018
Online since: 2019-04-11T11:58:31Z
Abstract: The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts.
Funding Details: Department of Agriculture, Food and the Marine
Type of material: Journal Article
Publisher: Elsevier
Journal: Experimental Parasitology
Volume: 187
Start page: 30
End page: 36
Copyright (published version): 2018 Elsevier
Keywords: PMA-qPCRPolymer adhesionFluorescence in-situ hybridizationExcystationPropidium iodide
DOI: 10.1016/j.exppara.2018.03.002
Language: en
Status of Item: Peer reviewed
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Appears in Collections:Veterinary Medicine Research Collection

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