Comparison of in vitro viability methods for Cryptosporidium oocysts
|Title:||Comparison of in vitro viability methods for Cryptosporidium oocysts||Authors:||Vande Burgt, Nathan H.; Auer, Agathe; Zintl, Annetta||Permanent link:||http://hdl.handle.net/10197/9922||Date:||Apr-2018||Online since:||2019-04-11T11:58:31Z||Abstract:||The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts.||Funding Details:||Department of Agriculture, Food and the Marine||Type of material:||Journal Article||Publisher:||Elsevier||Journal:||Experimental Parasitology||Volume:||187||Start page:||30||End page:||36||Copyright (published version):||2018 Elsevier||Keywords:||PMA-qPCR; Polymer adhesion; Fluorescence in-situ hybridization; Excystation; Propidium iodide||DOI:||10.1016/j.exppara.2018.03.002||Language:||en||Status of Item:||Peer reviewed||This item is made available under a Creative Commons License:||https://creativecommons.org/licenses/by-nc-nd/3.0/ie/|
|Appears in Collections:||Veterinary Medicine Research Collection|
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