Platelet extracellular vesicles induce a pro-inflammatory smooth muscle cell phenotype

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Title: Platelet extracellular vesicles induce a pro-inflammatory smooth muscle cell phenotype
Authors: Vajen, TanjaHeinzmann, Alexandra C. A.Parsons, Martin E.M.Maguire, Patricia al.
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Date: 16-May-2017
Online since: 2019-04-15T11:57:55Z
Abstract: Extracellular vesicles (EVs) are mediators of cell communication during health and disease, and abundantly released by platelets upon activation or during ageing. Platelet EVs exert modulatory effects on immune and vascular cells. Platelet EVs may modulate the function of vascular smooth muscle cells (SMC). Platelet EVs were isolated from platelet-rich plasma and incubated with SMC in order to assess binding, proliferation, migration and pro-inflammatory phenotype of the cells. Platelet EVs firmly bound to resting SMC through the platelet integrin αIIbβ3, while binding also occurred in a CX3CL1–CX3CR1-dependent manner after cytokine stimulation. Platelet EVs increased SMC migration comparable to platelet derived growth factor or platelet factor 4 and induced SMC proliferation, which relied on CD40- and P-selectin interactions. Flow-resistant monocyte adhesion to platelet EV-treated SMC was increased compared with resting SMC. Again, this adhesion depended on integrin αIIbβ3 and P-selectin, and to a lesser extent on CD40 and CX3CR1. Treatment of SMC with platelet EVs induced interleukin 6 secretion. Finally, platelet EVs induced a synthetic SMC morphology and decreased calponin expression. Collectively, these data indicate that platelet EVs exert a strong immunomodulatory activity on SMC. In particular, platelet EVs induce a switch towards a pro-inflammatory phenotype, stimulating vascular remodelling.
Funding Details: Netherlands Foundation for Scientific Research
Landsteiner Foundation for Blood Transfusion Research
Deutsche Forschungsgemeinschaft
Nutrim NWO Graduate Programme (Netherlands Foundation for Scientific Research)
Type of material: Journal Article
Publisher: Taylor & Francis
Journal: Journal of Extracellular Vesicles
Volume: 6
Issue: 1
Copyright (published version): 2017 the Authors
Keywords: Platelet factor 4CytokineSynthetic phenotypeVascular remodellingPathway analysisProteomicsCX3CR1
DOI: 10.1080/20013078.2017.1322454
Language: en
Status of Item: Peer reviewed
This item is made available under a Creative Commons License:
Appears in Collections:Conway Institute Research Collection
Biomolecular and Biomedical Science Research Collection

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