Thapa, Ganesh

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Thapa, Ganesh
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Thapa, Ganesh

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  • Publication
    Endurance assessment of Eichhornia crassipes (Mart.) Solms, in heavy metal contaminated site–A case study
    (Taylor & Francis, 2016-08-25)
    Thapa, Ganesh 
    ;
    Das, Debatosh 
    ;
    Gunupuru, Lokanadha R. 
    ;
    Tang, Bozeng 
    ;
    Speldewinde, Peter Christiaan 
    In this study, the ability to hyper accumulate heavy metals from contaminated site by water hyacinth (Eichhornia crassipes) was monitored. The heavy metal-contaminated habitat was validated by X-ray diffraction and energy dispersive X-ray elemental spectrometry analysis of the soil samples. Heavy metal contamination in soil and water; accumulation in foliar, root and bulb tissue samples were determined by atomic absorption spectroscopy and were monitored as a function of accumulation in different tissues. Significant differences were recorded in the bioaccumulation capability of heavy metals by different tissue. Discrete variation in protein profile of leaves and high expression of alcohol dehydrogenase (ADH), peroxidase (POX) and altered regulation of esterase (EST) in root tissue was observed in contaminated site grown Eichhornia. The high metal accumulation efficiency of water hyacinth due to the biomass production suggests this species as reliable organic biomarker for heavy metal contamination.
      147Scopus© Citations 8
  • Publication
    A wheat cytochrome P450 enhances both resistance to deoxynivalenol and grain yield
    (PLOS, 2018-10-12)
    Gunupuru, Lokanadha R. 
    ;
    Arunachalam, Chanemougasoundharam 
    ;
    Malla, Keshav B. 
    ;
    Kahla, Amal 
    ;
    Perochon, Alexandre 
    ;
    Jia, Jianguang 
    ;
    Thapa, Ganesh 
    ;
    Doohan, Fiona M. 
    The mycotoxin deoxynivalenol (DON) serves as a plant disease virulence factor for the fungi Fusarium graminearum and F. culmorum during the development of Fusarium head blight (FHB) disease on wheat. A wheat cytochrome P450 gene from the subfamily CYP72A, TaCYP72A, was cloned from wheat cultivar CM82036. TaCYP72A was located on chromosome 3A with homeologs present on 3B and 3D of the wheat genome. Using gene expression studies, we showed that TaCYP72A variants were activated in wheat spikelets as an early response to F. graminearum, and this activation was in response to the mycotoxic Fusarium virulence factor deoxynivalenol (DON). Virus induced gene silencing (VIGS) studies in wheat heads revealed that this gene family contributes to DON resistance. VIGS resulted in more DON-induced discoloration of spikelets, as compared to mock VIGS treatment. In addition to positively affecting DON resistance, TaCYP72A also had a positive effect on grain number. VIGS of TaCYP72A genes reduced grain number by more than 59%. Thus, we provide evidence that TaCYP72A contributes to host resistance to DON and conclude that this gene family warrants further assessment as positive contributors to both biotic stress resistance and grain development in wheat.
      294Scopus© Citations 40