Danish, Minna K.

Thumbnail Image
Preferred name
Danish, Minna K.
Official Name
Danish, Minna K.

Research Output

Filters

3
3
Reset filters

Settings

Now showing 1 - 3 of 3
  • Publication
    Formulation, Characterization and Stability Assessment of a Food‐Derived Tripeptide, Leucine‐Lysine‐Proline Loaded Chitosan Nanoparticles
    (Wiley, 2017-09)
    Danish, Minna K. 
    ;
    Vozza, Giuliana 
    ;
    Byrne, Hugh J. 
    ;
    Frías, Jesús M. 
    ;
    Ryan, Sinéad M. 
    The chicken‐ or fish‐derived tripeptide, leucine‐lysine‐proline (LKP), inhibits the angiotensin converting enzyme and may be used as an alternative treatment for prehypertension. However, it has low permeation across the small intestine. The formulation of LKP into a nanoparticle (NP) has the potential to address this issue. LKP‐loaded NPs were produced using an ionotropic gelation technique, using chitosan (CL113). Following optimization of unloaded NPs, a mixture amount design was constructed using variable concentration of CL113 and tripolyphosphate at a fixed LKP concentration. Resultant particle sizes ranged from 120 to 271 nm, zeta potential values from 29 to 37 mV, and polydispersity values from 0.3 to 0.6. A ratio of 6:1 (CL113:TPP) produced the best encapsulation of approximately 65%. Accelerated studies of the loaded NPs indicated stability under normal storage conditions (room temperature). Cytotoxicity assessment showed no significant loss of cell viability and in vitro release studies indicated an initial burst followed by a slower and sustained release.
      286Scopus© Citations 6
  • Publication
    Application of Box-Behnken experimental design for the formulation and optimisation of selenomethionine-loaded chitosan nanoparticles coated with zein for oral delivery
    (Elsevier, 2018-11-15)
    Vozza, Giuliana 
    ;
    Danish, Minna K. 
    ;
    Byrne, Hugh J. 
    ;
    Frías, Jesús M. 
    ;
    Ryan, Sinéad M. 
    Selenomethionine is an essential amino acid with a narrow therapeutic index and susceptibility to oxidation. Here it was encapsulated into a nanoparticle composed of chitosan cross-linked with tripolyphosphate for oral delivery. The formulation was optimised using a three-factor Box-Behnken experimental design. The chitosan:tripolyphosphate ratio, chitosan solvent pH, and drug load concentration were independently varied. The dependent variables studied were encapsulation efficiency, particle size, polydispersity index and zeta potential. For optimisation, encapsulation efficiency and zeta potential were maximised, particle diameter was set to 300 nm and polydispersity index was minimised. A 0.15 mg/mL concentration of selenomethionine, chitosan solvent pH of 3, and chitosan:tripolyphosphate ratio of 6:1 yielded optimum nanoparticles of size 187 ± 58 nm, polydispersity index 0.24 ± 0.01, zeta potential 36 ± 6 mV, and encapsulation efficiency of 39 ± 3%. Encapsulation efficiency was doubled to 80 ± 1.5% by varying pH of the ionotropic solution components and by subsequent coating of the NPs with zein, increasing NP diameter to 377 ± 47 nm, whilst retaining polydispersity index and zeta potential values. Selenomethionine-entrapped nanoparticles were not cytotoxic to intestinal and liver cell lines. Accelerated thermal stability studies indicated good stability of the nanoparticles under normal storage conditions (23 °C). In simulated gastrointestinal and intestinal fluid conditions, 60% cumulative release was obtained over 6 h.
      282Scopus© Citations 19
  • Publication
    Comparative study of the structural and physicochemical properties of two food derived antihypertensive tri-peptides, Isoleucine-Proline-Proline and Leucine-Lysine-Proline encapsulated into a chitosan based nanoparticle system
    (Elsevier, 2017-12)
    Danish, Minna K. 
    ;
    Vozza, Giuliana 
    ;
    Byrne, Hugh J. 
    ;
    Frías, Jesús M. 
    ;
    Ryan, Sinéad M. 
    Food derived tri-peptides; Leucine-Lysine-Proline (LKP) and Isoleucine-Proline-Proline (IPP) are angiotensin converting enzyme inhibitors and may have potential to alleviate hypertension. The aim of this work was to understand the interactions of IPP and LKP when formulated into a chitosan nanoparticle (NP) to help improve permeation. Our findings indicate different mean inhibitory concentrations (LKP: 0.36 ± 0.01 μM and IPP: 3.1 ± 0.6 μM) and encapsulation efficiencies at different ratios of chitosan: tripolyphosphate (LKP NPs: 65% at 6:1 and IPP NPs: 43% at 4:1). Molecular modelling and circular dichroism showed different stable amino side-chain-specific conformations for each peptide. IPP showed more steric hindrances to intra-chain rotations, resulting in an unordered peptide structure, whereas LKP showed more flexibility associated with a (disordered) β-strand-like conformer. In-vitro release kinetics showed a slower release for LKP NPs in acidic pH compared to IPP NPs. In conclusion, LKP NPs were found to have better binding compatibility with chitosan.
      421Scopus© Citations 12