Now showing 1 - 4 of 4
  • Publication
    Protein adhesion on water stable atmospheric plasma deposited acrylic acid coatings
    (Elsevier, 2013-11-15) ;
    There is considerable interest in the application of plasma polymerised acrylic acid (ppAAc) coatings due to their ability to enhance the adhesion of cells and proteins. An issue with this coating however is its stability in water and previous studies carried out using low pressure plasmas have demonstrated that high plasma powers are required to achieve water stable coatings. In this paper the use of both helium and air atmospheric plasmas is compared for the deposition of ppAAc coatings. The deposition studies were carried out on silicon wafer substrates using the PlasmaStream™ and PlasmaTreat™ plasma jet deposition systems respectively. The coatings were characterised using contact angle, FTIR, SEM, XPS, ellipsometry and optical profilometry. While both the helium and air plasmas were successful in the deposition of ppAAc coatings, the nm thick films deposited using the PlasmaTreat system exhibited significantly higher levels of water stability, probably due to a higher level of coating cross-linking. Ellipsometry measurements demonstrated only a 0.2 nm reduction in the thickness of an 18 nm thick ppAAc coating, when immersed in an aqueous buffer solution for one hour. Protein attachment studies were carried out using a flow cell system, which was monitored using a spectroscopic ellipsometer. This study was carried out with Bovine Serum Albumin (BSA), Immunoglobulin G (IgG) and Fibrinogen (Fg) proteins. In all three cases increased levels of protein adhesion were observed for the ppAAc coating, compared to that obtained on the uncoated silicon wafer substrates.
      996Scopus© Citations 29
  • Publication
    Protein Adhesion on Atmospheric Plasma Deposited Quaternary Ammonium Salt Coatings
    (Wiley Blackwell (John Wiley & Sons), 2013-03-19) ;
    This study investigates protein adhesion on nm thick helium atmospheric plasma deposited quaternary ammonium salt (QAS) coatings. The adhesion of the proteins BSA, IgG and Fg was evaluated on coated and uncoated silicon wafer substrates. This study was carried out in PBS solution, under flow conditions using ellipsometry. The QAS was found to exhibit a low level of solubility in PBS over time (approx. 2 nm h−1). On addition of both the IgG and Fg proteins, it was found that a protective protein layer of 7 and 2 nm respectively was formed, which prevented further dissolution of the QAS. In contrast the 1 nm thick BSA protein layer, which formed on the QAS, was insufficiently thick to prevent the slow dissolution of the salt. It was concluded that the charge and structure of the protein influences its adhesion on the QAS surface.
      577Scopus© Citations 3
  • Publication
    Acoustic Metrology : From Atmospheric Plasma to Solo Percussive Irish Dance
    LabVIEW software is used to decode step sequences generated by Irish light and hard shoes and bare feet. To remove the low frequency reverberation of the floor a Savitzky-Golay digital filter is used to de-convolute the percussion sound of the step sequences. Floor types and foot apparel are compared.
  • Publication
    Influence of coating properties on the adhesion of proteins to atmospheric plasma modified surfaces
    Protein adhesion is of key importance for the biocompatibility of medical devices. This study investigates the adsorption of protein, bovine serum albumin (BSA), onto both uncoated silicon wafers and nanometre thick fluorosiloxane coated wafers. A plasma polymerised coating was deposited from a mixture of tetramethylcyclotetrasiloxane (TC) and perfluorooctyltriethoxysilane (FS) (1:1 by vol. ratio). The liquid precursor mixture was nebulised into an atmospheric plasma jet formed using the PlasmaStreamTM system. The adsorption of protein on the plasma polymerised coatings was evaluated under dynamic flow conditions using a spectroscopic ellipsometry technique. The rate of protein adsorption onto coated and uncoated silicon wafer substrates was monitored over time after the BSA solution was introduced into a flow cell. These measurements indicated the adsorption of a 2 nm thick BSA protein layer on the uncoated silicon wafers. The ellipsometry thickness measurements of adsorbed protein on silicon wafer were confirmed using quartz crystal microbalance measurements (QCM). The BSA adsorption studies were then repeated with a fluorosiloxane coating. These coatings exhibited a highly textured surface morphology with low surface energy and a high water contact angle of 156 . The ellipsometry flow cell tests with BSA indicated almost no adsorption of protein onto the superhydrophobic fluorosiloxane coating. This study demonstrated the ability of ellipsometry to measure protein adsorption under dynamic flow 2 conditions and the influence of surface properties on protein adsorption.