Now showing 1 - 5 of 5
  • Publication
    Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface
    Nanoparticles have been proposed as carriers for drugs, genes and therapies to treat various diseases1, 2. Many strategies have been developed to target nanomaterials to specific or over-expressed receptors in diseased cells, and these typically involve functionalizing the surface of nanoparticles with proteins, antibodies or other biomolecules. Here, we show that the targeting ability of such functionalized nanoparticles may disappear when they are placed in a biological environment. Using transferrin-conjugated nanoparticles, we found that proteins in the media can shield transferrin from binding to both its targeted receptors on cells and soluble transferrin receptors. Although nanoparticles continue to enter cells, the targeting specificity of transferrin is lost. Our results suggest that when nanoparticles are placed in a complex biological environment, interaction with other proteins in the medium and the formation of a protein corona3, 4 can ‘screen’ the targeting molecules on the surface of nanoparticles and cause loss of specificity in targeting.
      3116Scopus© Citations 1481
  • Publication
    Intelligent middleware for adaptive sensing of tennis coaching sessions
    In professional tennis training matches, the coach needs to be able to view play from the most appropriate angle in order to monitor players activities. In this paper, we present a system which can adapt the operation of a series of cameras in order to maintain optimal system performance based on a set of wireless sensors. This setup is used as a testbed for an agent based intelligent middleware that can correlate data from many different wired and wireless sensors and provide effective in-situ decision making. The proposed solution is flexible enough to allow the addition of new sensors and actuators. Within this setup we also provide details of a case study for the embedded control of cameras through the use of Ubisense data.
      913Scopus© Citations 1
  • Publication
    Engineering ambient visual sensors
    Visual sensors are an indispensable prerequisite for those AmI environments that require a surveillance component. One practical issue concerns maximizing the operational longevity of such sensors as the operational lifetime of an AmI environment itself is dependent on that of its constituent components. In this paper, the intelligent agent paradigm is considered as a basis for managing a camera collective such that the conflicting demands of power usage optimization and system performance are reconciled.
      261
  • Publication
    In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation
    Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core-shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes.
      253Scopus© Citations 14
  • Publication
    Mapping protein binding sites on the biomolecular corona of nanoparticles
    Nanoparticles in a biological milieu are known to form a sufficiently long-lived and well-organized 'corona' of biomolecules to confer a biological identity to the particle. Because this nanoparticle-biomolecule complex interacts with cells and biological barriers, potentially engaging with different biological pathways, it is important to clarify the presentation of functional biomolecular motifs at its interface. Here, we demonstrate that by using antibody-labelled gold nanoparticles, differential centrifugal sedimentation and various imaging techniques it is possible to identify the spatial location of proteins, their functional motifs and their binding sites. We show that for transferrin-coated polystyrene nanoparticles only a minority of adsorbed proteins exhibit functional motifs and the spatial organization appears random, which is consistent, overall, with a stochastic and irreversible adsorption process. Our methods are applicable to a wide array of nanoparticles and can offer a microscopic molecular description of the biological identity of nanoparticles.
      555Scopus© Citations 299