Boselli, LucaLucaBoselliPolo, EsterEsterPoloCastagnola, ValentinaValentinaCastagnolaDawson, Kenneth A.Kenneth A.Dawson2017-12-012017-12-012017 the A2017-04-03Angewandte Chemiehttp://hdl.handle.net/10197/9070Ultrasmall nanoparticles (USNPs), usually defined as NPs with core in the size range 1–3 nm, are a class of nanomaterials which show unique physicochemical properties, often different from larger NPs of the same material. Moreover, there are also indications that USNPs might have distinct properties in their biological interactions. For example, recent in vivo experiments suggest that some USNPs escape the liver, spleen, and kidney, in contrast to larger NPs that are strongly accumulated in the liver. Here, we present a simple approach to study the biomolecular interactions at the USNPs bio-nanointerface, opening up the possibility to systematically link these observations to microscopic molecular principles.enThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Bioinorganic chemistryElectrophoresisNanoparticlesProteinsProteomicsJournal Article56154215421810.1002/anie.2017003432017-05-30https://creativecommons.org/licenses/by-nc-nd/3.0/ie/