Riccombeni, AlessandroAlessandroRiccombeniButler, GeraldineGeraldineButler2012-11-282012-11-282012 Sprin2012-09Current Fungal Infection Reports1936-3761http://hdl.handle.net/10197/3934Since its introduction in the last decade, massive parallel sequencing, or "next-generation sequencing", has revolutionized our access to genomic information, providing accurate data with increasingly higher yields and lower costs with respect to first-generation technology. Massive parallel sequencing of cDNA, or RNA-seq, is progressively replacing array-based technology as the method of choice for transcriptomics. This review describes some of the most recent applications of next-generation sequencing technology to the study of pathogenic fungi, including Candida, Aspergillus and Cryptococcusspecies. Several integrated approaches illustrate the power and accuracy of RNA-seq for studying the biology of human fungal pathogens. In addition, the lack of consistency in data analysis is discussed.enThe final publication is available at springerlink.comAspergillusBioinformaticsCandidaChIP-seqCryptococcusDermatophytesGenomicsNext-generation sequencingRNA-seqNucleotide sequenceGenomicsBioinformaticsAspergillusCandidaCryptococcusDermatophytesJournal ArticleSeptember 201210.1007/s12281-012-0104-z2012-09-19https://creativecommons.org/licenses/by-nc-nd/3.0/ie/