Muzard, JulienJulienMuzardPlatt, MarkMarkPlattLee, Gil U.Gil U.Lee2017-03-022017-03-022012 Wiley2012-08-06Smallhttp://hdl.handle.net/10197/8386The growth of the biopharmaceutical industry has created a demand for new technologies for the purification of genetically engineered proteins.The efficiency of large-scale, high-gradient magnetic fishing could be improved if magnetic particles offering higher binding capacity and magnetization were available. This article describes several strategies for synthesizing microbeads that are composed of a M13 bacteriophage layer assembled on a superparamagnetic core. Chemically cross-linking the pVIII proteins to a carboxyl functionalized bead produced highly responsive superparamagnetic particles (SPM) with a side-on oriented, adherent virus monolayer. Also, the genetic manipulation of the pIII proteins with a His6 peptide sequence allowed reversible assembly of the bacteriophage on a nitrilotriacetic acid functionalized core in an end-on configuration. These phage-magnetic particles were successfully used to separate antibodies from high-protein concentration solutions in a single step with a > 90 % purity. The dense magnetic core of these particles makes themfive times more responsive to magnetic fields than commercial materialscomposed of polymer-iron oxide compositesand a monolayer of phage could produced a 1000 fold higher antibody binding capacity. These new bionanomaterials appear to be well-suited to large-scale high-gradient magnetic fishing separation and promise to be cost effective as a result of the self-assembling and self-replicating properties of genetically engineered M13 bacteriophageenThis is the author's version of the following article: Julien Muzard, Mark Platt, Gil Lee (2012) "Affinity Separation: M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation" 8(15) which has been published in final form at http://dx.doi.org/10.1002/smll.201290083.BionanomaterialM13 filamentous phageAffinity separationDownstream processingSuperparamagnetic beadsHigh gradient magnetic separationJournal Article81510.1002/smll.2012900832017-01-24https://creativecommons.org/licenses/by-nc-nd/3.0/ie/