Reid, Helen M.Helen M.ReidWikström, KatarinaKatarinaWikströmKavanagh, David J.David J.KavanaghMulvaney, Eamon P.Eamon P.MulvaneyKinsella, B. ThereseB. ThereseKinsella2011-09-212011-09-212011 Elsev2011-04Cell Signalling0898-6568http://hdl.handle.net/10197/3161In humans, thromboxane (TX) A2 signals through the TPα and TPβ isoforms of its G-protein coupled TXA2 receptor (TP) to mediate a host of (patho)physiologic responses. Herein, angio-associated migratory cell protein (AAMP) was identified as a novel interacting partner of both TPα and TPβ through an interaction dependent on common (residues 312-328) and unique (residues 366-392 of TPβ) sequences within their carboxyl-terminal (C)-tail domains. While the interaction was constitutive in mammalian cells, agonist-stimulation of TPα/TPβ led to a transient dissociation of AAMP from immune complexes which coincided with a transient redistribution of AAMP from its localization in an intracellular fibrous network. Although the GTPase RhoA is a downstream effector of both AAMP and the TPs, AAMP did not influence TP-mediated RhoA or vice versa. Small interfering RNA (siRNA)-mediated disruption of AAMP expression decreased migration of primary human coronary artery smooth muscle cells (1° hCoASMCs). Moreover, siRNA-disruption of AAMP significantly impaired 1° hCoASMC migration in the presence of the TXA2 mimetic U46619 but did not affect VEGF-mediated cell migration. Given their roles within the vasculature, the identification of a specific interaction between TPα/TPβ and AAMP is likely to have substantial functional implications for vascular pathologies in which they are both implicated.8152227 bytesapplication/pdfenThis is the author’s version of a work that was accepted for publication in Cellular Signalling. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cellular Signalling Volume 23, Issue 4, April 2011, Pages 700-717 DOI 10.1016/j.cellsig.2010.12.003.ThromboxaneReceptorAngio-associated migratory cell proteinCell migrationInteractantYeast two hybridRhoAThromboxanesCell receptorsCell migrationG proteinsThromboxanesReceptors, Thromboxane A2, Prostaglandin H2AAMP protein, humanrhoA GTP-Binding ProteinCell MovementTwo-Hybrid System TechniquesJournal Article23470071710.1016/j.cellsig.2010.12.003https://creativecommons.org/licenses/by-nc-sa/1.0/