Clark, Benjamin R.Benjamin R.ClarkMurphy, Cormac D.Cormac D.Murphy2013-11-122013-11-122009, Roya2008-10-31Organic and Biomolecular Chemistryhttp://hdl.handle.net/10197/4911The biosynthesis of the pyrrolylpolyene rumbrin (1) in the fungus Auxarthron umbrinum was elucidated using feeding studies with labelled precursors. Incorporation of stable isotopes from [15N]-proline, [13C]-methionine and [13C]-acetate confirmed that these were the precursors of the pyrrole moiety, methyl groups, and backbone of rumbrin, respectively. Label-dilution experiments with pyrrole-2-carboxylate confirmed it was a direct precursor in the biosynthesis of rumbrin. Both 3- and 4-chloropyrrolecarboxylates were also accepted as precursors in polyene production.enPolyene productionJournal Article7111111610.1039/b813236d2013-10-03https://creativecommons.org/licenses/by-nc-nd/3.0/ie/