Quaglia, DanielaDanielaQuagliaGalletti, PaolaPaolaGallettiEmer, EnricoEnricoEmerParadisi, FrancescaFrancescaParadisiGiacomini, DariaDariaGiacomini2013-06-202013-06-202013 Elsev2013-05Process Biochemistryhttp://hdl.handle.net/10197/4376The novel histidine-tagged Horse Liver Alcohol Dehydrogenase (His-HLADH-EE) was successfully purified and covalently immobilized onto a solid support in a one-step procedure through a metal-directed technique. A full characterization of the immobilized enzyme was carried out. Effects of pH, temperature and organic co-solvents were deeply investigated and they showed a shift in the optimum pH with respect to the free form as well as increased stability to temperature and solvents. The immobilized His-HLADH-EE proved to be effective as catalyst in the reduction of aliphatic and aromatic aldehydes. Application of the free and immobilized His-HLADH-EE to the chemo-enzymatic synthesis of (S)-Profenols demonstrated enhanced enantioselectivity and high reusability of the immobilized form. The achievement of a robust and effective immobilization of an alcohol dehydrogenase substantiated the use of biocatalytic reduction in the synthesis of primary alcohols and valuable chiral intermediates especially for pharmaceutical industries.enThis is the author’s version of a work that was accepted for publication in Process Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Process Biochemistry (Volume 48, Issues 5–6, May–June 2013, Pages 810–818) DOI:10.1016/j.procbio.2013.03.016 Elsevier Ltd.Alcohol dehydrogenasesImmobilizationEnantioselective reductionProfenolsDynamic kinetic resolutionHorse liver alcohol dehydrogenaseJournal Article485-681081810.1016/j.procbio.2013.03.0162013-06-10https://creativecommons.org/licenses/by-nc-nd/3.0/ie/