Yarrow, FionaFionaYarrowRice, James H.James H.Rice2013-08-062013-08-062011, Spri2011-02European Biophysics Journal With Biophysics Lettershttp://hdl.handle.net/10197/4502IR absorption spectroscopy of hemoglobin was performed using an infrared (IR) optical parametric oscillator laser and a commercial atomic force microscope (AFM) in a novel experimental arrangement based on the use of a bottom-up excitation alignment. This experimental approach enables detection of protein samples with resolution much higher than that of standard IR spectroscopy. Presented here are AFM-based IR absorption spectra of micron-sized hemoglobin features.enThe final publication is available at www.springerlink.comNano-IRInfrared absorptionNanoimagingNanoscopyImagingChemical mappingAFMJournal Article40221721910.1007/s00249-010-0634-72013-07-04https://creativecommons.org/licenses/by-nc-nd/3.0/ie/