Nagy, ZoltanZoltanNagyComer, ShaneShaneComerSmolenski, Albert P.Albert P.Smolenski2021-11-102021-11-102018 Wiley2018-07-25Current Protocols in Protein Science1934-3655http://hdl.handle.net/10197/12617Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn2+ -Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application.Print-ElectronicenThis is the peer reviewed version of the following article: Nagy, Z., Comer, S., & Smolenski, A. (2018). Analysis of protein phosphorylation using Phos‐tag gels. Current Protocols in Protein Science, 93, e64. which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/cpps.64ZincPyridinesPhosphoproteinsphosphate‐affinity electrophoresisPhosphorylationJournal Article93110.1002/cpps.642020-09-1811/TIDA/B2021RG/15/13/31673https://creativecommons.org/licenses/by-nc-nd/3.0/ie/