Investigation of facilitative urea transporters in the human gastrointestinal tract

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Title: Investigation of facilitative urea transporters in the human gastrointestinal tract
Authors: Walpole, Caragh
McGrane, Alison
Al-mousawi, Hashemeya
Winter, Desmond C.
Baird, Alan W.
Stewart, Gavin
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Date: 12-Aug-2018
Online since: 2019-04-24T10:35:21Z
Abstract: The symbiotic relationship between humans and their intestinal microbiomeis supported by urea nitrogen salvaging. Previous studies have shown thatcolonic UT-B urea transporters play a significant role in this important physi-ological process. This current study investigated UT-A and UT-B urea trans-porter expression along the human gastrointestinal tract. Initial end-pointPCR experiments determined that UT-A RNA was predominantly expressedin the small intestine, while UT-B RNA was expressed in stomach, small intes-tine, and colon. Using western blotting experiments, a strong 40–60 kDa UT-B signal was found to be abundant in both ileum and colon. Importantly, thissignal was deglycosylated by PNGaseF enzyme treatment to a core protein of30 kDa in both tissues. Further immunolocalization studies revealed UT-Btransporter proteins were present at the apical membrane of the villi in theileum, but predominantly at the basolateral membrane of the colonic surfaceepithelial cells. Finally, a blind scoring immunolocalization study suggestedthat there was no significant difference in UT-B abundance throughout thecolon (NS, ANOVA,N=5–21). In conclusion, this current study suggestedUT-B to be the main human intestinal urea transporter. Intriguingly, thesedata suggested that the same UT-B isoform was present in all intestinalepithelial cells, but that the precise cellular location varied.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Journal: Physiological Reports
Volume: 6
Issue: 15
Start page: e13826
Copyright (published version): 2018 the Authors
Keywords: Gastrointestinal tractHumanUT‐AUT‐B
DOI: 10.14814/phy2.13826
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Biology & Environmental Science Research Collection

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