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Linking long-term soil phosphorus management to microbial communities involved in nitrogen reactions
Date Issued
2022-02-24
Date Available
2022-10-21T11:24:07Z
Abstract
The influence of soil phosphorous (P) content on the N-cycling communities and subsequent effects on N2O emissions remains unclear. Two laboratory incubation experiments were conducted on soils collected from a long-term (est. 1995) P-addition field trial sampled in summer 2018 and winter 2019. Incubations were treated with a typical field amendment rate of N as well as a C-amendment to stimulate microbial activity. Throughout both incubations, soil subsamples were collected prior to fertiliser amendment and then throughout the incubations, to quantify the abundance of bacteria (16S rRNA), fungi (ITS) and Thaumarcheota (16S rRNA) as well as functional guilds of genes involved in nitrification (bacterial and archaeal amoA, and comammox) and denitrification (nirS, nirK, nosZ clade I and II) using quantitative PCR (qPCR). We also evaluated the correlations between each gene abundance and the associated N2O emissions depending on P-treatments. Our results show that long-term P-application influenced N-cycling genes abundance differently. Except for comammox, overall nitrifiers’ genes were most abundant in low P while the opposite trend was found for denitrifiers’ genes. C and N-amendments strongly influenced the abundance of most genes with changes observed as soon as 24 h after application. ITS was the only gene correlated to N2O emissions in the low P-soils while microbes were mostly correlated to emissions in high P, suggesting possible changes in the organisms involved in N2O production depending on soil P-content. This study highlights the importance of long-term P addition on shaping the microbial community function which in turn stimulates a direct impact on the subsequent N emissions.
Sponsorship
Department of Agriculture, Food and the Marine
Teagasc
Other Sponsorship
Open Access funding provided by IReL
Type of Material
Journal Article
Publisher
Springer
Journal
Biology and Fertility of Soils
Volume
58
Issue
4
Start Page
389
End Page
402
Copyright (Published Version)
2022 The Authors
Language
English
Status of Item
Peer reviewed
ISSN
0178-2762
This item is made available under a Creative Commons License
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Name
O'neill et al. 2022.pdf
Size
1.36 MB
Format
Adobe PDF
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