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Delayed differentiation of vaginal and uterine microbiomes in dairy cows developing postpartum endometritis
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Miranda-CasoLuengo et al. - 2019 - Delayed differentiation of vaginal and uterine mic.pdf | 3.58 MB |
Date Issued
10 January 2019
Date Available
21T11:07:09Z May 2019
Abstract
Bacterial overgrowth in the uterus is a normal event after parturition. In contrast to the healthy cow, animals unable to control the infection within 21 days after calving develop postpartum endometritis. Studies on the Microbial Ecology of the bovine reproductive tract have focused on either vaginal or uterine microbiomes. This is the first study that compares both microbiomes in the same animals. Terminal Restriction Fragment Length Polymorphism of the 16S rRNA gene showed that despite large differences associated to individuals, a shared community exist in vagina and uterus during the postpartum period. The largest changes associated with development of endometritis were observed at 7 days postpartum, a time when vaginal and uterine microbiomes were most similar. 16S rRNA pyrosequencing of the vaginal microbiome at 7 days postpartum showed at least three different microbiome types that were associated with later development of postpartum endometritis. All three microbiome types featured reduced bacterial diversity. Taken together, the above findings support a scenario where disruption of the compartmentalization of the reproductive tract during parturition results in the dispersal and mixing of the vaginal and uterine microbiomes, which subsequently are subject to differentiation. This differentiation was observed early postpartum in the healthy cow. In contrast, loss of bacterial diversity and dominance of the microbiome by few bacterial taxa were related to a delayed succession at 7DPP in cows that at 21 DPP or later were diagnosed with endometritis.
Sponsorship
Department of Agriculture, Food and the Marine
Science Foundation Ireland
Type of Material
Journal Article
Publisher
PLOS
Journal
PLoS ONE
Volume
14
Issue
1
Start Page
e0200974
Copyright (Published Version)
2019 the Authors
Language
English
Status of Item
Peer reviewed
ISSN
1932-6203
This item is made available under a Creative Commons License
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